The Mini Extruder Pro is a compact, easy-to-use tool that allows researchers to prepare uniform unilamellar vesicles (liposomes) using a controlled extrusion process. It is designed for efficiency, reliability, and flexibility across a wide range of lipid compositions..jpg?la=en-GB&hash=02AA02E7E3C272DDFBCCCFFD97D5102E)
Convenient
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Quickly prepare unilamellar vesicles through a simple extrusion process
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Easy to assemble, operate, and clean
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All wetted parts can be cleaned rapidly, minimizing downtime between lipid formulations
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Produces uniform vesicles without solvents or detergents
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Vesicle size is easily controlled by selecting the appropriate polycarbonate membrane
Affordable
The Mini Extruder Pro is constructed of stainless steel and Teflon; the new design will provide years of trouble-free service and is available for a fraction of the cost of alternative options.
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Constructed from durable stainless steel and Teflon for long‑term, trouble‑free use
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Available at a fraction of the cost of alternative options
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Replacement parts are readily available from Avanti Research
Complete set includes:
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Mini Extruder Pro body
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Stainless steel extruder outer casing
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PTFE Internal Membrane Support (2)
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O-ring (2)
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Stainless steel Retainer Nut
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Base
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2 gas-tight 1mL syringes
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100 0.1µm polycarbonate membranes
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100 filter supports
Flexible
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Can be placed directly on a hot plate for rapid temperature control
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Base acts as a heating block allowing for extrusion at elevated temperatures. Ideal for lipids with phase transition temperatures above room temperature
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Capable of producing unilamellar liposomes ranging from ~30 nm to 5 µm, depending on formulation composition and membrane selection
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Compatible with a wide variety of lipid compositions
Reliable Performance
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Vesicle size distribution depends on the number of passes through the membrane
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Hydrated lipids initially form large, multilamellar vesicles
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With repeated extrusion: Early passes yield a broad or bimodal size distribution
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Additional passes yield a narrow, uniform particle size distribution
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At least 11 passes through the membrane are recommended for most lipid systems
Hydrated lipid will initially form large, multilamellar vesicles. After the initial pass through a membrane, the particle size distribution will tend towards bimodal. After sufficient passes through the membrane, a unimodal, normal distribution is obtained. A minimum of eleven passes through the extruder membrane is recommended for most lipids.
Placing the heating block assembly on a hot plate achieves rapid temperature control for lipids that have transition temperatures above room temperature.
Products
Instructions
- Assembly Detailed instructions on assembly of the Avanti Mini-Extruder
- Extrusion Detailed instructions on extrusion of liposomes
- Cleaning Detailed instructions on cleaning of syringes