4-HNE-dimethylacetal 870608 (E)-4R-hydroxynonenal-dimethylacetal (Each vial contains 6.8mg which yields ~5.2mg 4-HNE after deprotection)
4-Hydroxynonenal (4-HNE or HNE) is an α,β-unsaturated hydroxyalkenal which is produced by lipid peroxidation in cells. 4-HNE has been hypothesized by several researchers to play a key role in cell signal transduction, in a variety of pathways from cell cycle events to cellular adhesion.
The dimethylacetal derivative is a more stable form of 4-HNE which is good for long term storage of the compound during use. It can be easily converted to 4-HNE in the presence of acid (see deprotection procedure).
Each vial contains 6.8mg which yields ~5.2mg 4-HNE after deprotection.
Deprotection Procedure
- Open glass ampoule and transfer the contents of the ampoule to the Teflon vial (included) using a glass pipet or syringe. DO NOT USE PLASTIC.
- Evaporate the hexane under a gentle stream of nitrogen at room temperature. Keep lipid residue at 4°C for deprotection procedure.
- Add 1ml of 1mM HCl (4°C) and stir for about 45 minutes or close the vial and shake for 30 minutes.
- The suspension is initially cloudy and will become almost clear as deprotection occurs.
- The resulting aqueous HNE solution has a pH of 3 and contains ~5.2mg of aldehyde.
- This solution, if kept at 4°C, is stable for at least 24 hours.
- If neutralized (see note below), this product is stable for 6 hours.
- Once deprotected, we recommend that HNE be used immediately.
Note: HNE decomposes (polymerizes) in basic solution within a few minutes. Never use alkalies to neutralize the pH, but rather appropriately concentrated buffers. During saponification, methanol is generated. If methanol is undesirable, HNE can be extracted in chloroform or dichloromethane. HNE is only sparingly soluble in water.
