Brain PI(4,5)P2 840046 L-α-phosphatidylinositol-4,5-bisphosphate (Brain, Porcine) (ammonium salt)

Brain phosphatidylinositol 4,5-bisphosphate (Brain PI(4,5)P₂) is a low-abundance signaling naturally occurring phospholipid, isolated from porcine brain and supplied in ammonium salt forms (CAS 383907-42-4). It serves as a key plasma-membrane lipid that supports receptor-driven signaling, ion channel regulation, and cytoskeletal organization. It can be converted by phospholipase C into IP₃ and DAG or by class I PI3Ks into PI(3,4,5)P₃ to drive downstream pathways, including the activation of protein kinase AKT.

Brain-derived PI(4,5)P₂ preserves native acyl-chain heterogeneity and is well-suited for liposome preparation, reconstituted membrane systems, binding assays, and protein–lipid interaction studies requiring a physiologically relevant phosphoinositide environment.

Due to its high negative charge and signaling potency, PI(4,5)P2 is typically incorporated at low mol% in model membranes. Fatty acid distribution varies by source/batch (see “fatty acid distribution” table below).

Applications

• Protein–lipid interaction assays, including PH-, ENTH-, and PX-domain binding and membrane recruitment studies.
• Enzymatic and reconstitution studies of PLC-mediated hydrolysis and PI3K-dependent phosphorylation pathways.
• Incorporation into model membrane systems (LUVs, GUVs, supported lipid bilayers) for biophysical and membrane dynamics investigations.
• In vitro trafficking and cytoskeletal regulation studies where PI(4,5)P₂ contributes to membrane identity and protein localization.

Formulation & handling

Available from Avanti Research™ in ammonium salt form, as a powder or dissolved in organic solvent. For optimal stability, store at −20 °C, protected from light and moisture. Allow material to equilibrate to room temperature before opening to avoid condensation.