300216 | LNP Lipid Blend 5
LNP Lipid Blend 5
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LNP Lipid Blend 5
LNP Lipid Blend 5
This pre-mixed lipid blend is composed of components suitable for producing nucleic acid-based lipid nanoparticles. Works best with mRNA cargo, and can be used for other nucleic acids as well.
Instructions for using the LNP Lipid Blend 5:
1. Add 1-mL of ethanol to the vial to achieve 25 mg/mL. Mix well to achieve full dissolution.*
2. Dilute nucleic acid in aqueous buffer.**
3. Use solvent injection to create LNPs. Different parameters and cargo will result in different particle size results.
4. Dialyze the final formulation against buffer of choice to remove residual ethanol.
| Catalog Number | Component Name | Mole Fraction |
| 890899 | DODMA | 50 |
| 700107 | Sitosterol (synthetic from plant sterol) | 38.5 |
| 850365 | DSPC | 10 |
| 880151 | DMG-PEG2000 | 1.5 |
*Note: low heat, sonication, or vortexing can be used as needed
**Note: common buffers include citrate, Tris, or PBS
Powered by Bioz- Certificate of Analysis (Lot No. 300216P-1EA-A-010 and 7097PAA010)
- Certificate of Analysis (Lot No. 300216P-1EA-A-010 and 7097PAA010)